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nrg1β1  (R&D Systems)


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    R&D Systems nrg1β1
    Evaluation of Rgs16 expression in acute and chronic injury and after <t>NRG1β1</t> stimulation of Schwann cells. (A–C) Quantitative expression analysis of Rgs16 under regenerating and degenerating conditions following traumatic injury to the median nerve in Wistar rats. The relative quantification of Rgs16 was evaluated by qRT-PCR at different time points after injury (d = day post-injury). Regenerating conditions: (A) crush injury; (B) end-to-end repair after transection injury. Degenerating conditions: (C) transection injury. The geometric mean of the housekeeping genes Ankrd27 and Rictor was used to normalize data. The healthy nerves were used as calibrator. The values in the graphs are expressed as the mean ± SEM (n = 3-6 each group). (D) Comparative analysis of Rgs gene expression in rat Schwann cells after chronic constriction injury obtained from single-cell RNA sequencing dataset. The heatmap shows average expression levels of the Rgs gene family for uninjured (Un) versus 3 days post-injury (3d) and 12 days post-injury (12d) conditions. The table below presents adjusted p-values from Wilcoxon and Likelihood-ratio (bimodal) tests for Rgs16 , indicating significant differential expression. (E, F) Normalized counts of RNA-sequencing data of Rgs16 and Nrg1 genes in a model of chronic demyelinating pathology Charcot-Marie-Tooth disease type-1A during development. The values in the graphs are expressed as the mean ± SEM (n = 4 each group). (G) Quantitative expression analysis by qRT-PCR of Rgs16 after 10 nM NRG1β1 stimulation of Schwann cells. Tbp was used as a housekeeping gene to normalize data. Untreated cells were used as calibrator. The values in the graph are expressed as the mean ± SEM (n = 3 each group). (H) Western blot analysis of RGS16 after 10 nM NRG1β1 stimulation of primary cultures of Schwann cells at different time points.
    Nrg1β1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 126 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+nrg1%CE%B21/Recombinant+Human+NRG1-beta+1%2FHRG1-beta+1+EGF+Domain+Protein/pmc11983618-158-39-41
    Average 95 stars, based on 126 article reviews
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    1) Product Images from "Dynamic regulation of Rgs16 and its correlation with Neuregulin1 expression in acute and chronic nerve injury"

    Article Title: Dynamic regulation of Rgs16 and its correlation with Neuregulin1 expression in acute and chronic nerve injury

    Journal: Frontiers in Cell and Developmental Biology

    doi: 10.3389/fcell.2025.1540453

    Evaluation of Rgs16 expression in acute and chronic injury and after NRG1β1 stimulation of Schwann cells. (A–C) Quantitative expression analysis of Rgs16 under regenerating and degenerating conditions following traumatic injury to the median nerve in Wistar rats. The relative quantification of Rgs16 was evaluated by qRT-PCR at different time points after injury (d = day post-injury). Regenerating conditions: (A) crush injury; (B) end-to-end repair after transection injury. Degenerating conditions: (C) transection injury. The geometric mean of the housekeeping genes Ankrd27 and Rictor was used to normalize data. The healthy nerves were used as calibrator. The values in the graphs are expressed as the mean ± SEM (n = 3-6 each group). (D) Comparative analysis of Rgs gene expression in rat Schwann cells after chronic constriction injury obtained from single-cell RNA sequencing dataset. The heatmap shows average expression levels of the Rgs gene family for uninjured (Un) versus 3 days post-injury (3d) and 12 days post-injury (12d) conditions. The table below presents adjusted p-values from Wilcoxon and Likelihood-ratio (bimodal) tests for Rgs16 , indicating significant differential expression. (E, F) Normalized counts of RNA-sequencing data of Rgs16 and Nrg1 genes in a model of chronic demyelinating pathology Charcot-Marie-Tooth disease type-1A during development. The values in the graphs are expressed as the mean ± SEM (n = 4 each group). (G) Quantitative expression analysis by qRT-PCR of Rgs16 after 10 nM NRG1β1 stimulation of Schwann cells. Tbp was used as a housekeeping gene to normalize data. Untreated cells were used as calibrator. The values in the graph are expressed as the mean ± SEM (n = 3 each group). (H) Western blot analysis of RGS16 after 10 nM NRG1β1 stimulation of primary cultures of Schwann cells at different time points.
    Figure Legend Snippet: Evaluation of Rgs16 expression in acute and chronic injury and after NRG1β1 stimulation of Schwann cells. (A–C) Quantitative expression analysis of Rgs16 under regenerating and degenerating conditions following traumatic injury to the median nerve in Wistar rats. The relative quantification of Rgs16 was evaluated by qRT-PCR at different time points after injury (d = day post-injury). Regenerating conditions: (A) crush injury; (B) end-to-end repair after transection injury. Degenerating conditions: (C) transection injury. The geometric mean of the housekeeping genes Ankrd27 and Rictor was used to normalize data. The healthy nerves were used as calibrator. The values in the graphs are expressed as the mean ± SEM (n = 3-6 each group). (D) Comparative analysis of Rgs gene expression in rat Schwann cells after chronic constriction injury obtained from single-cell RNA sequencing dataset. The heatmap shows average expression levels of the Rgs gene family for uninjured (Un) versus 3 days post-injury (3d) and 12 days post-injury (12d) conditions. The table below presents adjusted p-values from Wilcoxon and Likelihood-ratio (bimodal) tests for Rgs16 , indicating significant differential expression. (E, F) Normalized counts of RNA-sequencing data of Rgs16 and Nrg1 genes in a model of chronic demyelinating pathology Charcot-Marie-Tooth disease type-1A during development. The values in the graphs are expressed as the mean ± SEM (n = 4 each group). (G) Quantitative expression analysis by qRT-PCR of Rgs16 after 10 nM NRG1β1 stimulation of Schwann cells. Tbp was used as a housekeeping gene to normalize data. Untreated cells were used as calibrator. The values in the graph are expressed as the mean ± SEM (n = 3 each group). (H) Western blot analysis of RGS16 after 10 nM NRG1β1 stimulation of primary cultures of Schwann cells at different time points.

    Techniques Used: Expressing, Quantitative Proteomics, Quantitative RT-PCR, Gene Expression, RNA Sequencing, Western Blot

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    Article Title: NMDA Receptors Regulate Neuregulin 2 Binding to ER-PM Junctions and Ectodomain Release by ADAM10 [corrected].
    Article Snippet: AP5, BACE-IV, heparinase I–III enzymes, and Complete protease inhibitor cocktail were from Millipore Sigma. .. Recombinant human NRG1β1 (EGFlike domain) was from R&D systems (HB-396). .. Constructs All expression constructs were generated using Gateway cloning (Thermo Fisher) from open reading frames cloned into entry vectors pENTR223.1 (NRG2) or pENTR/D (NRG1, ADAM10, and ADAM17) that were transferred by recombination to Gateway destination vector pAAV (hSynI), a variant of pAAV_MCS (Agilent) driving cDNA expression from the human synapsin I promoter.

    Article Title: NMDA receptors regulate Neuregulin 2 binding to ER-PM junctions and ectodomain release
    Article Snippet: AP5, BACE-IV, Heparinase I-III enzymes and CompleteTM protease inhibitor cocktail were from Millipore Sigma. .. Recombinant human NRG1β1 (EGF-like domain) was from R&D systems (HB-396). ..



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    Evaluation of Rgs16 expression in acute and chronic injury and after <t>NRG1β1</t> stimulation of Schwann cells. (A–C) Quantitative expression analysis of Rgs16 under regenerating and degenerating conditions following traumatic injury to the median nerve in Wistar rats. The relative quantification of Rgs16 was evaluated by qRT-PCR at different time points after injury (d = day post-injury). Regenerating conditions: (A) crush injury; (B) end-to-end repair after transection injury. Degenerating conditions: (C) transection injury. The geometric mean of the housekeeping genes Ankrd27 and Rictor was used to normalize data. The healthy nerves were used as calibrator. The values in the graphs are expressed as the mean ± SEM (n = 3-6 each group). (D) Comparative analysis of Rgs gene expression in rat Schwann cells after chronic constriction injury obtained from single-cell RNA sequencing dataset. The heatmap shows average expression levels of the Rgs gene family for uninjured (Un) versus 3 days post-injury (3d) and 12 days post-injury (12d) conditions. The table below presents adjusted p-values from Wilcoxon and Likelihood-ratio (bimodal) tests for Rgs16 , indicating significant differential expression. (E, F) Normalized counts of RNA-sequencing data of Rgs16 and Nrg1 genes in a model of chronic demyelinating pathology Charcot-Marie-Tooth disease type-1A during development. The values in the graphs are expressed as the mean ± SEM (n = 4 each group). (G) Quantitative expression analysis by qRT-PCR of Rgs16 after 10 nM NRG1β1 stimulation of Schwann cells. Tbp was used as a housekeeping gene to normalize data. Untreated cells were used as calibrator. The values in the graph are expressed as the mean ± SEM (n = 3 each group). (H) Western blot analysis of RGS16 after 10 nM NRG1β1 stimulation of primary cultures of Schwann cells at different time points.
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    Evaluation of Rgs16 expression in acute and chronic injury and after <t>NRG1β1</t> stimulation of Schwann cells. (A–C) Quantitative expression analysis of Rgs16 under regenerating and degenerating conditions following traumatic injury to the median nerve in Wistar rats. The relative quantification of Rgs16 was evaluated by qRT-PCR at different time points after injury (d = day post-injury). Regenerating conditions: (A) crush injury; (B) end-to-end repair after transection injury. Degenerating conditions: (C) transection injury. The geometric mean of the housekeeping genes Ankrd27 and Rictor was used to normalize data. The healthy nerves were used as calibrator. The values in the graphs are expressed as the mean ± SEM (n = 3-6 each group). (D) Comparative analysis of Rgs gene expression in rat Schwann cells after chronic constriction injury obtained from single-cell RNA sequencing dataset. The heatmap shows average expression levels of the Rgs gene family for uninjured (Un) versus 3 days post-injury (3d) and 12 days post-injury (12d) conditions. The table below presents adjusted p-values from Wilcoxon and Likelihood-ratio (bimodal) tests for Rgs16 , indicating significant differential expression. (E, F) Normalized counts of RNA-sequencing data of Rgs16 and Nrg1 genes in a model of chronic demyelinating pathology Charcot-Marie-Tooth disease type-1A during development. The values in the graphs are expressed as the mean ± SEM (n = 4 each group). (G) Quantitative expression analysis by qRT-PCR of Rgs16 after 10 nM NRG1β1 stimulation of Schwann cells. Tbp was used as a housekeeping gene to normalize data. Untreated cells were used as calibrator. The values in the graph are expressed as the mean ± SEM (n = 3 each group). (H) Western blot analysis of RGS16 after 10 nM NRG1β1 stimulation of primary cultures of Schwann cells at different time points.
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    Evaluation of Rgs16 expression in acute and chronic injury and after <t>NRG1β1</t> stimulation of Schwann cells. (A–C) Quantitative expression analysis of Rgs16 under regenerating and degenerating conditions following traumatic injury to the median nerve in Wistar rats. The relative quantification of Rgs16 was evaluated by qRT-PCR at different time points after injury (d = day post-injury). Regenerating conditions: (A) crush injury; (B) end-to-end repair after transection injury. Degenerating conditions: (C) transection injury. The geometric mean of the housekeeping genes Ankrd27 and Rictor was used to normalize data. The healthy nerves were used as calibrator. The values in the graphs are expressed as the mean ± SEM (n = 3-6 each group). (D) Comparative analysis of Rgs gene expression in rat Schwann cells after chronic constriction injury obtained from single-cell RNA sequencing dataset. The heatmap shows average expression levels of the Rgs gene family for uninjured (Un) versus 3 days post-injury (3d) and 12 days post-injury (12d) conditions. The table below presents adjusted p-values from Wilcoxon and Likelihood-ratio (bimodal) tests for Rgs16 , indicating significant differential expression. (E, F) Normalized counts of RNA-sequencing data of Rgs16 and Nrg1 genes in a model of chronic demyelinating pathology Charcot-Marie-Tooth disease type-1A during development. The values in the graphs are expressed as the mean ± SEM (n = 4 each group). (G) Quantitative expression analysis by qRT-PCR of Rgs16 after 10 nM NRG1β1 stimulation of Schwann cells. Tbp was used as a housekeeping gene to normalize data. Untreated cells were used as calibrator. The values in the graph are expressed as the mean ± SEM (n = 3 each group). (H) Western blot analysis of RGS16 after 10 nM NRG1β1 stimulation of primary cultures of Schwann cells at different time points.
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    Evaluation of Rgs16 expression in acute and chronic injury and after NRG1β1 stimulation of Schwann cells. (A–C) Quantitative expression analysis of Rgs16 under regenerating and degenerating conditions following traumatic injury to the median nerve in Wistar rats. The relative quantification of Rgs16 was evaluated by qRT-PCR at different time points after injury (d = day post-injury). Regenerating conditions: (A) crush injury; (B) end-to-end repair after transection injury. Degenerating conditions: (C) transection injury. The geometric mean of the housekeeping genes Ankrd27 and Rictor was used to normalize data. The healthy nerves were used as calibrator. The values in the graphs are expressed as the mean ± SEM (n = 3-6 each group). (D) Comparative analysis of Rgs gene expression in rat Schwann cells after chronic constriction injury obtained from single-cell RNA sequencing dataset. The heatmap shows average expression levels of the Rgs gene family for uninjured (Un) versus 3 days post-injury (3d) and 12 days post-injury (12d) conditions. The table below presents adjusted p-values from Wilcoxon and Likelihood-ratio (bimodal) tests for Rgs16 , indicating significant differential expression. (E, F) Normalized counts of RNA-sequencing data of Rgs16 and Nrg1 genes in a model of chronic demyelinating pathology Charcot-Marie-Tooth disease type-1A during development. The values in the graphs are expressed as the mean ± SEM (n = 4 each group). (G) Quantitative expression analysis by qRT-PCR of Rgs16 after 10 nM NRG1β1 stimulation of Schwann cells. Tbp was used as a housekeeping gene to normalize data. Untreated cells were used as calibrator. The values in the graph are expressed as the mean ± SEM (n = 3 each group). (H) Western blot analysis of RGS16 after 10 nM NRG1β1 stimulation of primary cultures of Schwann cells at different time points.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Dynamic regulation of Rgs16 and its correlation with Neuregulin1 expression in acute and chronic nerve injury

    doi: 10.3389/fcell.2025.1540453

    Figure Lengend Snippet: Evaluation of Rgs16 expression in acute and chronic injury and after NRG1β1 stimulation of Schwann cells. (A–C) Quantitative expression analysis of Rgs16 under regenerating and degenerating conditions following traumatic injury to the median nerve in Wistar rats. The relative quantification of Rgs16 was evaluated by qRT-PCR at different time points after injury (d = day post-injury). Regenerating conditions: (A) crush injury; (B) end-to-end repair after transection injury. Degenerating conditions: (C) transection injury. The geometric mean of the housekeeping genes Ankrd27 and Rictor was used to normalize data. The healthy nerves were used as calibrator. The values in the graphs are expressed as the mean ± SEM (n = 3-6 each group). (D) Comparative analysis of Rgs gene expression in rat Schwann cells after chronic constriction injury obtained from single-cell RNA sequencing dataset. The heatmap shows average expression levels of the Rgs gene family for uninjured (Un) versus 3 days post-injury (3d) and 12 days post-injury (12d) conditions. The table below presents adjusted p-values from Wilcoxon and Likelihood-ratio (bimodal) tests for Rgs16 , indicating significant differential expression. (E, F) Normalized counts of RNA-sequencing data of Rgs16 and Nrg1 genes in a model of chronic demyelinating pathology Charcot-Marie-Tooth disease type-1A during development. The values in the graphs are expressed as the mean ± SEM (n = 4 each group). (G) Quantitative expression analysis by qRT-PCR of Rgs16 after 10 nM NRG1β1 stimulation of Schwann cells. Tbp was used as a housekeeping gene to normalize data. Untreated cells were used as calibrator. The values in the graph are expressed as the mean ± SEM (n = 3 each group). (H) Western blot analysis of RGS16 after 10 nM NRG1β1 stimulation of primary cultures of Schwann cells at different time points.

    Article Snippet: Cells were centrifuged at 100 rcf for 5 min and then resuspended in a selective DMEM D-valine medium (AL251-500ML; HiMedia Laboratories, Thane, India) enriched with 10% FBS, 100 μg/mL streptomycin, 100 U/mL penicillin, 10 μM forskolin and 8 nM NRG1β1 (#396-HB, R&D Systems, Minneapolis, United States) and plated onto poly-L-lysine (PLL) coated culture dishes.

    Techniques: Expressing, Quantitative Proteomics, Quantitative RT-PCR, Gene Expression, RNA Sequencing, Western Blot